How to Remove Air Bubbles from HPLC Columns - uHPLCs By employing a combination of these methods, you can effectively detect air bubbles and take steps to eliminate them before they compromise your HPLC analysis
Remove air bubbles from columns to restore flow rate Degassing or removing dissolved air from all samples and buffers before applying them to a column will minimize or eliminate problems associated with the presence of air bubbles Degas solutions by subjecting them to vacuum for several minutes (see related Tech Tip procedure)
# 1 Most Common Problem in HPLC: Air Bubble in the Pump Simple Fixing Method: We demonstrate a straightforward procedure to eliminate air bubbles, including opening the purge valve and adjusting the flow rate Verifying Solution Effectiveness:
Air bubble in HPLC Pump - Chromatography Forum First of all please clean the inlet frit of your solvent connected to pump B Than disconnect your column and guard column and purge your HPLC system with an organic solvent That should eliminate the air in your HPLC system
What is the best way to remove air from the solvent lines of . . . Turn the handle counterclockwise, about 1 2-turn, to open the valve, and pull back on the plunger to remove air bubbles Once the air is removed, close the valve and start flow Flush the system with 100% methanol for a minimum of 30 minutes