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- Primer designing tool - National Center for Biotechnology Information
Enter an organism name (or organism group name such as enterobacteriaceae, rodents), taxonomy id or select from the suggestion list as you type Help Entrez query (optional) Help Primer specificity stringency Primer must have at least 1 2 3 4 5 6 total mismatches to unintended targets, including
- National Center for Biotechnology Information
Try "re-search for specific primers" feature on Primer-BLAST result page When only non-specific primers are found, the result page offers the option to re-search for specific primers
- Primer-Blast results
Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target
- Primer-Blast results - National Center for Biotechnology Information
Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target
- Primer-BLAST: a tool to design target-specific primers for . . . - PubMed
We describe a robust and fully implemented general purpose primer design tool that designs target-specific PCR primers Primer-BLAST offers flexible options to adjust the specificity threshold and other primer properties
- CREPE (CREate Primers and Evaluate): a computational tool for large . . .
Its final output summarizes the lead forward and reverse primer pair for each target site, a measure of the likelihood of binding to off-targets, and additional information to aid decision-making
- Primer-BLAST Archives - NCBI Insights
To find primers for a group of related sequences, Primer-BLAST aligns the longest sequence to the rest to find common regions It uses these to limit the locations of primers The longest sequence is also used as the representative template sequence in the results
- Primer, Pipelines, Parameters: Issues in 16S rRNA Gene Sequencing
Primer choice influences the estimated microbial composition A set of different 16S rRNA gene sequencing primer pairs covering one, two, or three of the variable regions V1 to V9 is commonly used for the analysis of microbial compositions
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