NIBSC - Influenza potency testing Single radial immunodiffusion (SRD) The SRD test for inactivated influenza vaccines was developed at NIBSC in the 1970s This assay measures the quantity of haemagglutinin (HA) antigen present in a sample – such as a vaccine – using an immunological reaction between antibodies and the test antigen
VaxArray assessment of influenza split vaccine potency and stability (SRD aka SRID) measures the immunological reaction between antisera and test antigen and is the only current internationally recognized method for establishing and tracking influenza vaccine potency and stability [4]
A novel method for automating zone measurement of SRD assays - Synbiosis A novel method for automating zone measurement of SRD assays The principle behind single radial immunodiffusion (SRD) relies on the incorporation of an antibody specific for the analytes being measured into an agar gel as the gel is being poured
Single Radial Immunodiffusion - SpringerLink Single radial immunodiffusion is used extensively for the quantitative estimation of antigens (1) In this method, the antigen-antibody precipitation is made more sensitive than in double immunodiffusion (see Chapter 135) by the incorporation of the antiserum in the
Assaying the Potency of Influenza Vaccines - MDPI The potency of influenza vaccines has been successfully assayed by single radial immunodiffusion (SRID or SRD) for decades Every year the circulating influenza viruses are reviewed and the strains included in the vaccines updated as necessary
Reversed Single-Radial-Immunodiffusion Test: The Method for the Assay . . . During an epidemic of H3N2 and H1N1 viruses, the r-SRD test was employed to estimate the infection ratio in junior high school students The results demonstrated its convenience and high sensitivity in detecting the antibody rise to influenza A nucleoprotein
A single radial immunodiffusion test for antibodies to newcastle . . . A single radial immunodiffusion (SRD) test for the measurement of anti-bodies to Newcastle disease virus (NDV) is described The test involved incorporation of concentrated intact NDV into a gel of agarose with low setting temperature and measurement of opalescent zones produced by
Analytical method validation: A brief review - World Journal of . . . Analytical method validation includes the determination of accuracy, precision, LOD, LOQ, linearity and range The results from method validation can be used to moderator the quality, reliability and consistency of analytical results, which is an integral part of any good analytical practice